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biochem jm all that is biochem will be discussed here more info info button biochem jm post find yourself june 12 2015 leave a reply who am i what is my purpose these are very important questions which require deep reflection most importantly be honest to yourself truly listen it will protect you from giving into the stereotypes and expectations that the world imposes on you spend time answering these questions now you do not want to over commit to a path that is not for you you may have heard someone confess i really did not want to be __________ but instead wanted to become ____ sure you may achieve some level of success but if you don t establish your identity you would not have fulfilled your true potential as you look back in the twilight of your career what will be your answers to these questions will i do it over the same way again have i wasted these years now more than ever we need bold creative and innovative individuals our future depends on it jm post how to write up the measurement of arginase activity lab october 24 2014 leave a reply please read the lab manual general guidelines document on myelearning for general instructions on how to write up the lab here are some pointers for this particular lab id name of lab partner title date the date you did the lab aim be very specific what were you measuring name of enzyme what was the source of the enzyme introduction this is a year 2 lab you don t have to define what is an enzyme or show how it works focus on the enzyme you are working with all enzymes will have an ec number start there what information does the digits in the ec number give where in the body is the enzyme located organ as well as subcellular level state its reaction show that it is part of the urea cycle describe the principle of the assay used rxn of urea with inpp show structures note you don t have to talk about common laboratory equipment such as the spectrophotometer methodology and materials materials reagents must be listed with their concentrations where possible equipment glassware not necessary summary of method continuous prose no point form past tense no tables the full method has to be given don t say as seen in the manual you are expected to be able to use the method in your lab report and be able to do the lab without referring to the manual results you must state your actual results if there is something wrong with your values then you can go see ms fariyal and get some theoretical results to do your calculations you will have to examine in your discussion why you think your results deviated from what was expected number and title your tables and graphs in addition to the table for your calibration curve you will want to tabulate your results for the 2 mixtures for your calibration curve state a title clearly labeled axes give a scale and it s the best straight line through the origin you can draw your graph on excel just set it up so that the straight line passes through the origin and get an equation of the line from excel so that you could work out the amount of urea in your samples calculations this was discussed during your lab session don t forget to calculate your corrected absorbances in addition you are required to do up a flow chart for your calculations this is not the flow chart you did for your lab session that broke down the procedure this flow chart is different and shows all the dilutions and aliquots taken this will let us know that you properly understood what you did in your calculations so you will start with the liver giving its mass and work you way down to the final aliquot stating volumes along the way discussion compare your results obtained with other theoretical experimental results discuss how enzyme activity is measured and compare these techniques with the one you did for this lab state sources of error and precautions taken for this expt why was the liver extract kept cold why was the reaction carried out at 37 o c why was mnso 4 used why were the tubes foiled the use of perchloric acid references use proper format as outlined in the chicago manual of style 16 th edition doc on myelearning your information in the introduction and discussion must be cited and a list of your references given in alphabetical order at the end diagrams and figures reproduced must be referenced other things to note remember its ml not ml attached a signed accountability statement form to your lab submit your lab a week from when the lab was done you are to submit your lab by 3 30pm to the biochem office rm 217 in the old nat sci wing submit your introduction and discussion in a word document to the turnitin link on myelearning this should be obvious but you will need to submit the same intro and discussion that you submitted from your hard copy to turnitin failure to submit to turnitin will result in loss of marks plagiarism will result in the loss of marks and depending on the level of plagiarism you will get a zero grade and reported to the uwi examinations board please direct all your questions to ms fariyal concerning the lab thank you jm post biochemistry extra credit assignment metabolomics october 19 2014 47 comments omics industries genomics transcriptomics proteomics metabolomics these have become core technologies within almost every major academic or industrial research program around the world when choosing a msc or phd programme you should ensure that they are covering some if not all of the above if you want to remain scientifically relevant and marketable in the future metabolomics is a field of life science research that uses high throughput technologies to identify and or characterize all the small molecules or metabolites in a given cell tissue or organism i e the metabolome metabolomics is a very young area of research as we now have the ability to measure and characterize very small molecules metabolites in an organism most common analytical techniques used in metabolomics are gas chromatography mas spectrometry gc ms liquid chromatography mas spectrometry lc ms nuclear magnetic resonance nmr spectroscopy your mission discuss how metabolomics studies have contributed to our understanding of disease mechanisms and the implications this will have on improving or possibly providing novel treatment strategies for these diseases your reply could be in the form of a blog post reply or a video reply if you are using text then a minimum of 250 words should be used and for videos the time limit is 5 mins please reference your material and include links to useful information please state clearly in your reply the course code either your name or id suggestion do up your reply as a post for your blog or video for your youtube channel and then post that link up in the reply section of my blog you are required to submit the text of your post or transcript of your video in a word document to the turnitin link on myelearning only responses made in the reply section of this blog and submitted to turnitin will be marked deadline 30 th nov a maximum of 10 marks will be awarded based on the quality of your answer please be reminded that this is an extra credit assignment and not a compulsory requirement for this course please let me know if you have any questions jm post biochemistry extra credit assignment supplements october 18 2014 80 comments supplements are everywhere dietary supplements and bodybuilding supplements proteins amino acids cofactors hormones fat burners antioxidants and multivitamins these supplements are being sold based on what we know they do naturally in the human body how efficacious these isolated purified and sometimes synthesized supplements are is a discussion for another time your mission i want you to chose any supplement and in the comments section of this blog post the following a picture of the supplement state what the manufacturer is claiming this supplement is doing by identifying the active ingredient s in the supplement justify the manufacturer s claims using your biochemical knowledge of human metabolism be creative with your responses it could be in the form of a blog post reply or a video reply if you are using text then a minimum of 250 words should be used and for videos the time limit is 5 mins please state clearly in your reply the course code either your name or id suggestion do up your reply as a post for your blog or video for your youtube channel and then post that link up in the reply section of my blog you are required to submit the text of your post or transcript of your video in a word document to the turnitin link on myelearning only responses made in the reply section of this blog and submitted to turnitin will be marked deadline 28 th nov a maximum of 10 marks will be awarded based on the quality of your answer please be reminded that this is an extra credit assignment and not a compulsory requirement for this course please let me know if you have any questions jm post principles of therapeutics and drug action pointers for biol 3363 october 11 2014 leave a reply biol 3363 medical biotech topic principles of therapeutics and drug action these are some things i want you all to look at when studying this topic know your definitions agonist anaphylactic antagonist bioavailability which mode of administration will give you 100 bioavailability efficacy permeability coefficient pharmacodynamics pharmacokinetics potency prophylactic therapeutic therapeutic index tolerance differentiate between pharmacodynamics and pharmacokinetics the mechanisms of different types of receptors ligand gated channels study the nicotinic receptor and the mechanism of action of acetylcholine ach on it g protein coupled receptor use the mechanism of action of glucagon as the example g protein coupled receptors tend to have the same general mechanism so we can use glucagon as the example however different g proteins have different second messengers associated with them i want you to name 4 second messengers kinase linked receptors use the mechanisms of action of insulin as the example note the difference between peptide hormones and steroid hormones you should be able to describe the mechanism of action of cardiac gylycosides digitalis note when talking about mechanisms of action you want to describe the structure of the receptor and the sequence of events that follow when agonist bind to receptor please use the blog to share information with everyone by posting up comments links and questions in the comments section below post game birds can t fly far october 3 2014 leave a reply did you know that game birds such as pheasants and quail are easily fatigued and can t fly long distances this is especially true when they are startled a strategy used by many hunters the reason being that that the flight muscles of game birds rely almost entirely on the metabolism of glycogen stored in the muscle it begs the question how do migratory birds generate energy to fly long distances love to know what you think post things to know for fst 2014 2015 registration august 16 2014 1 comment hey guys just got this from the deputy dean very important things to know for registration just click on the link below to get the pdf fst registration 2014 cheers jm post glycolysis may 22 2014 leave a reply chimmeral so there is this special biochemical process right and it is universal meaning that it takes place in all living cells from the smallest prokaryote to the largest eukayoric cell to exist that is awesome it is also one of the most ancient biochemical processes to exist so it even occurred in the first life form to ever appear on the face of the earth what do you think this process os called well if you guessed glycoloysis you are correct according to david l nelson and michael m cox glycoloysis is the catabolic pathway by which a molecule of glucose is broken down into two molecules of pyruvate so what happens in glycoloysis well view original post 2 231 more words post glycolysis inhibition for anticancer treatment may 22 2014 leave a reply chimmeral glycolysis inhibition for anticancer treatment link http www nature com onc journal v25 n34 abs 1209597a html review through understanding the methods in which cancer achieves the energy for cellular function new concepts to fight the disease can be drawn cancer generally utilizes an abnormal metabolic pathway devoid or with little use of the tca and etc aspects of respiration whether the environment is hypoxic or not low oxygen availability cancer is often linked to an increase in aerobic glycolysis independent of the mitochondria s function which may be an adaptation due to the hypoxic conditions created with in a tumor as a result to a lack of angiogenesis or linked to damaged mitochondria mtdna in many cases malignant tumors may be completely reliant on glycolysis to produce the necessary atp and follow the lactic fermentation pathway this was noted in malignant cell even when oxygen levels were adequate for aerobic respiration this phenomenon was 1 st describe by otto warburg in view original post 246 more words post enzymes learn with a song may 21 2014 leave a reply biochemuwi1362 enzyme song uwi biochemistry 1362 let s see what we have to do enzymes enzymes let s see what s inside of you enzymes are proteins made up of amino acids like glycine they are important to us catalyse a reaction without fuss they mash up the place with no change to their structure even within range they are very specific to their substrate and yes they affect the rate they lower the activation energy and enable us in this country and the whole world to see and be a living being like you and me chorus no matter what we eat we still are made of meat with proteins that live inside making up all them enzymes verse 2 you are wondering by now why enzymes do this and how enzymes have an active site substrates do not fit if they re not right during the reaction they bind to the site in view original post 103 more words older blog at wordpress com biochem jm blog at wordpress com subscribe subscribed biochem jm join 381 other subscribers sign me up already have a wordpress com account log in now privacy biochem jm subscribe subscribed sign up log in report this content view site in reader manage subscriptions collapse this bar loading comments write a comment email required name required website