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medicine for all medicine for all medicine for all medicine for all skip to content home medicine for all contact older posts optimal oocyte retrieval and embryo transfer techniques part 5 posted on 12 april 2022 by azaleaazelia approaches of oocyte recovery in the early days of ivf experimentation abdominal laporatomy was performed to collect oocytes during tubal ligation procedures surgery would be scheduled immediately prior to expected ovulation timed by basal body temperatures follicles were extracted by needle aspiration or ovarian wedge resection techniques described for follicle aspiration involved puncturing follicles over 5 mm in diameter with a 20 gauge needle the aspiration needle was connected to tubing and emptied into a test tube through a metal inlet with a rubber stopper sealing the tube a second needle was introduced through the stopper and connected to surgical suction tubing via a three way plastic connector aspiration was achieved by covering the free opening in the three way connector to create suction to 200 mmhg each follicle was aspirated into individual tubes the techniques described for wedge resection involved incising follicles with a scalpel under microscopy subsequently releasing the oocytes into the culture medium for further observation using these techiques these reported oocyte recovery rate was 30 2 for aspiration and 93 3 for wedge resection although these techniques are a viable option to obtain oocytes laparotomies carry surgical risks to include bleeding infection increased pain potential injury to surrounding pelvic and abdominal organs and longer recovery times the risks of laparotomy to retrieve oocytes for ivf encouraged the pursuit of alternative surgical options posted in tak berkategori 1 comment optimal oocyte retrieval and embryo transfer techniques part 4 posted on 12 april 2022 by azaleaazelia approaches of oocyte recovery oocyte recovery aims to maximise the number of oocytes extracted from the ovarian follicles while minimizing the surgical risk to the patient optimizing this balance has driven the evolution of oocyte recovery techniques posted in tak berkategori leave a comment optimal oocyte retrieval and embryo transfer techniques part 3 posted on 11 april 2022 by azaleaazelia in the nearly 40 years since the first successful human birth art has undergone continuous improvement significant advances have been made in oocyte fertilization and embryo culture resulting in ever increased treatment success often overlooked however have been the developments in oocyte recovery and embryo transfer techniques that have led to increased safety and success of art treatment this document sets out to review the history oocyte recovery and embryo transfer techniques and to provide recommendations for current best practices posted in tak berkategori leave a comment optimal oocyte retrieval and embryo transfer techniques part 2 posted on 11 april 2022 by azaleaazelia the history of assisted reproductive technology art dates back more than a century when walter heape a professor at the university of cambridge reported the first case of embryo transfer in rabbits in 1890s several decades later aldous huxley conceptualized the technique of in vitro fertilization ivf in his science fiction novel brave new world c 1932 after successful mammalian experiments attempts at human ivf began in 1940s when miriam menken and john rock exposed human oocytes recovered via laparotomy to spermatozoa in vitro three decades later louise brown was born after successful ivf posted in tak berkategori leave a comment optimal oocyte retrieval and embryo transfer techniques part 1 posted on 10 april 2022 by azaleaazelia oocyte retrieval is most safely accomplished with conscious sedation via a transvaginal approach under ultrasound guidance with low pressure aspiration follicle flushing has not been shown to improve outcomes as the final step in embryo transfer is essential based on current findings the recommended embryo transfer technique includes the use of an ultrasound guided transcervical approach with a full bladder using a soft catheter the transfer depth of the embryo should be in the mid portion of the uterus with a quick steady insertion followed by pressure on the syringe during withdrawal of the catheter it is acceptable to encourage immediate ambulation after embryo transfer posted in tak berkategori leave a comment in vitro fertilization basics part 44 posted on 9 april 2022 by azaleaazelia decisions embryo freezing unless you have ethical objections they recommend you request to freeze any good quality spare embryos they culture spare embryos to day 5 so that only embryos that have demostrated their ability to keep developing are frozen posted in tak berkategori leave a comment in vitro fertilization basics part 43 posted on 9 april 2022 by azaleaazelia decisions at what stage to transfer embryos embryos are usually transferred on day 3 or day 5 after egg collection if there are several embryos of similar quality on day 3 then culturing to day 5 gives better information on which is the best embryo to transfer posted in tak berkategori leave a comment in vitro fertilization basics part 42 posted on 9 april 2022 by azaleaazelia decisions how many embryos to transfer the important facts to keep in mind are up to and including the age of 40 the chance of twins with two embryos back is around 25 30 between the ages of 41 and 44 the chance of twins with two embryos is around 10 15 even with one embryo back 2 of pregnancies are identical twins and even identical triplets have been reported with modern embryo freezing methods 90 95 of embryos survive freezing and thawing when the embryo survives freezing and thawing its chance of pregnancy is very similar to that of a fresh embryo posted in tak berkategori leave a comment in vitro fertilization basics part 41 posted on 8 april 2022 by azaleaazelia decisions how many embryos to transfer a woman s body is designed to carry one baby at a time as we mentioned before twins are associated with 2 3 times more risk for both the mother and child for a broad range of adverse outcomes from maternal death still birth to cerebral palsy transfer of one embryo is now standard for women 40 years and younger and strongly recommended for women 41 and older single embryo transfer is required for almost all publicly funded treatment posted in tak berkategori leave a comment in vitro fertilization basics part 40 posted on 7 april 2022 by azaleaazelia decisions you and your doctor will need to make several decisions about your in vitro fertilization cycle before you start treatment there are recorded in the doctor s management plan and those with an ethical aspect are also recorded in your consent form how many eggs to add sperm to on average 75 of mature eggs fertilize normally in in vitro fertilization and icsi unless you have an ethical objection to discarding poor quality embryos or to freezing spare embryos posted in tak berkategori leave a comment older posts archives archives select month april 2022 march 2022 february 2022 january 2022 december 2021 november 2021 october 2021 september 2021 august 2021 july 2021 june 2021 april 2021 march 2021 february 2021 december 2020 november 2020 october 2020 september 2020 august 2020 july 2020 june 2020 may 2020 april 2020 march 2020 february 2020 january 2020 december 2019 november 2019 october 2019 september 2019 august 2019 july 2019 june 2019 may 2019 april 2019 march 2019 february 2019 january 2019 december 2018 november 2018 october 2018 september 2018 august 2018 july 2018 june 2018 april 2018 march 2018 february 2018 january 2018 november 2017 september 2017 may 2017 april 2017 blog stats 145 157 hits search for medicine for all blog at wordpress com medicine for all create a free website or blog at wordpress com subscribe subscribed medicine for all join 2 296 other subscribers sign me up already have a wordpress com account log 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