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rhatikan tulisan populer berbeda dengan tulisan ilmiah yang cenderung kaku dan fokus berisi data data tulisan populer harus menghantarkan data dan fakta tersebut dengan bahasa dan cara yang mudah dicerna oleh sebagian besar lapisan masyarakat perlu diingat bahwa tulisan tidaklah bertujuan untuk menunjukkan kepintaran penulis namun bagaimana informasi dan ide itu tersampaikan pada pembaca ketiga sisi subjektif penulis juga perlu ditonjolkan dalam tulisan pemikiran perenungan dan tanggapan penulis terhadap suatu ide muncul dalam tulisan tulisan merupakan sudut pandang penulis dalam mengungkapkan ide penulis menuangkan hasil observasinya terhadap suatu peristiwa atau keadaan ke dalam tulisannya tulisan populer berbeda dengan text book yang cenderung kaku dan datar hanya memunculkan fakta dan data ilmiah keempat tulisan yang menarik akan memperbesar peluang tulisan anda merebut waktu pembaca tulisan anda harus atraktif itulah kata kunci untuk membuat rating tulisan anda menjadi tinggi begitu banyaknya tulisan yang ada di muka bumi ini membuat tulisan anda harus bersaing berebut pembaca layaknya iklan makanan di tv terkadang tulisan yang sedikit berbumbu garing rangginan akan cukup menarik bagi pembaca berikan kata kata dan kalimat yang layak untuk dibaca oleh pembaca bawakan tulisan dengan mengalir dan senantiasa membangkitkan rasa ingin tahu pembaca untuk terus membaca dari satu paragraf ke paragraf berikutnya berikan ide ide yang boom atau buzzword disetiap paragrafnya berikan pembaca alasan bahwa mereka layak meluangkan waktu mereka untuk membaca tulisan anda kelima tulisan juga harus menghibur pembaca akan bosan dan jenuh jika tulisan miskin bumbu bumbu hambar rasanya tulisan perlu ditambah bumbu lelucon guyonan segar bugar sehingga pembaca merasa terhibur dan senang dengan tulisan tersebut berikan lelucon atau guyonan cerdas dalam tulisan anda sedikit joke tidak akan membunuh tulisan jika tulisannya adalah tentang sesuatu yang mengharukan maka tulisan yang membangkitkan sisi emosional pembaca itulah yang menjadi titik tekannya terakhir buatlah tulisan dengan metode bercerita bertutur jangan menggunakan bahasa yang komplek kalimat panjang dengan banyak sub clause akan membuat pembaca mengernyitkan dahi naon sih ieu asa lieuer pembaca perlu mencerna lebih jauh terhadap kalimat panjang dan komplek komplek rumah mahal harganya sedangkan kalimat komplek bisa membuat tulisan anda menjadi murah karena ditinggal pembaca gunakanlah kalimat pendek namun jelas dan kaya informasi metode bercerita itu juga bersifat persuasif atau mengajak orang melakukan sesuatu itulah beberapa tips untuk menulis saya merangkumnya dalam kata kisah cerita yaitu singkatan dari kreatif informatif subjektif atraktif hiburan dan cerita tips tersebut cukup handal dalam membuat suatu tulisan populer dan tulisan ilmiah populer sedangkan untuk tulisan ilmiah memiliki karakter yang berbeda dengan populer tulisan ilmiah lebih fokus pada penyampaian data data menjadi penulis bukanlah pekerjaan mudah membutuhkan komitmen yang kuat tantangan dan halangan datang silih berganti konsistensi menulis sering terhalangi oleh kesibukan dan keengganan meng observasi dan belajar dari ilmu dan tulisan yang telah ada tentunya tulisan bukanlah sekedar tulisan tulisan yang baik adalah yang datang dari penelitian perenungan pengamatan pembacaan pemikiran yang menyeluruh oleh karena itulah menulis juga membutuhkan komitmen seperti para samurai yang senantiasa memegang teguh komitmen dalam sejarah samurai selalu memegang teguh komitmen bagi mereka kematian bukanlah hal terburuk yang dapat terjadi tragedi terbesar bagi mereka adalah menjalani hidup tanpa komitmen prinsip itulah spirit bushido selamat menulis selamat berjihad keluar dari budaya primitif para samurai pena posted by arif rahman s at 02 32 no comments friday 23 january 2009 rna bee rna isolation reagent overview catalog no cs 104b 100 ml cs 105b 200 ml cs 501b 500 ml storage store at 2 8 c product description rna bee is a complete and ready to use reagent for isolation of total rna from samples of human animal plant bacterial and viral origin rna bee is the improved version of the single step method of rna isolation 1 the improved rna bee provides a fast and highly reliable method for isolating pure and undegraded rna from a large variety of biological samples rna bee and the single step method are subjects of the us patent 4 843 155 rna bee is a monophase solution containing phenol and quanidine thiocyanate a biological sample is homogenized or lysed in rna bee and the homogenate lysate is separated into aqueous and organic phase by the addition of chloroform the subsequent centrifugation efficiently removes dna and proteins from the aqueous phase containing rna the undegraded pure rna is obtained from the aqueous phase by the isopropanol precipitation washing with ethanol and solubilization in an appropriate solution the entire isolation procedure can be completed in 1 hour the isolated rna is appropriate for northern blotting poly a selection rt pcr and other molecular biology techniques stability rna bee is stable at 2 8 c for at least one year from date of purchase special handling precautions rna bee contains a poison phenol and an irritant guanidine thiocyanate causes burns can be fatal when working with rna bee use gloves and eye protection face shield safety goggles do not get on skin or clothing avoid breathing fumes read the warning note on the container and msds in case of contact immediately flush eyes or skin with a large amount of water for at least 15 minutes and seek medical attention i protocol for rna isolation reagents required but not supplied chloroform isopropanol and ethanol we recommend the use of disposable polypropylene tubes the tubes should be tested to ensure integrity during centrifugation at 12 000g with rna bee and chloroform the protocol describes isolation of rna with 1 ml of rna bee using the following steps 1 homogenization 1 ml rna bee 50 mg tissue or 5 x 10 6 cells 2 phase separation homogenate 0 2 ml chloroform 3 rna precipitation aqueous phase 0 5 ml isopropanol 4 rna wash 1 ml 75 ethanol 5 rna solubilization water 0 5 sds or buffer all steps can be carried out at room temperature unless otherwise stated 1 homogenization a tissues homogenize tissue samples in rna bee 1 ml 50 mg tissue using a glass glass glass teflon or polytron homogenizer the sample volume should not exceed 10 of the rna bee volume b cells cells grown in monolayer should be lysed directly in the culture dish by the addition of rna bee use at least 1 ml of the reagent for a 3 5 cm petri dish pass the lysate through a pipette several times to ensure lysis cells grown in suspension should be sedimented first and then lysed by the addition of rna bee add at least 0 2 ml of rna bee per 10 6 cells and lyse by repeated pipetting 2 phase separation add 0 2 ml chloroform per 1 ml of rna bee cap the tube and shake vigorously for 15 30 seconds store the sample on ice or at least 4 c for 5 minutes centrifuge the homogenate at 12 000g for 15 minutes at 4 c following centrifugation the sample forms the lower blue phenol chloroform phase interphase and the upper colorless aqueous phase rna remains exclusively in the aqueous phase whereas dna and proteins are in the interphase and organic phase the volume of the aqueous phase is about 50 of the initial volume of rna bee plus sample volume chloroform should not contain isoamyl alcohol or any other additives 3 rna precipitation transfer the aqueous phase to a clean tube add 0 5 ml of isopropanol and store the sample for 5 10 minutes at room temperature centrifuge at 12 000g for 5 minutes at 4 25 c rna precipitate often not visible before centrifugation forms a white yellow pellet at the bottom of the tube 4 rna wash remove the supernatant and washs the rna pellet once with 75 ethanol shaking or votexing to dislodge the pellet from the side of the tube centrifuge for 5 minutes at 7 500g at 4 25 c use at least 1 ml of ethanol solution per 1 ml of rna bee used for the initial homogenization an additional wash with 75 ethanol improves 260 280 ratio and might be necessary to use the isolated rna in enzymatic assays 5 rna solubilization at the end of the procedure briefly air dry the rna pellet 5 10 minutes it is important not to let the rna pellet dry completely as this greatly decreases its solubility do not dry rna by centrifugation under vacuum dissolve the rna in water 0 5 sds or buffer by passing the solution through a pipette ip and or incubating for 10 15 minutes at 55 60 c tubes water or solutions used for rna solubilization should be made rnase free by diethyl pyrocarbonate depc treatment the final preparation of rna has a 260 280 ratio 1 6 1 9 posted by arif rahman s at 04 13 no comments tuesday 2 december 2008 purification genomic dna from soil_throughing method harnpicharnchai et al 2007 one among numerous problem from soil dna genomic is the present of inhibitors to remove them try this technique simple cheap and clean no need to use commercial kit 1 subject unpurified genomic dna from soil to electrophoresis in tae or tbe agarose gel 0 8 1 in the presence of 0 1 0 5 μg ml of ethidium bromide at 85 volts for approximately 45 minutes 2 make a rectangular well trough approximately 0 5 1 cm wide just below the dna band directly in front of the path of dna migration by a sharp scalpel 3 remove excess agarose in the well fill well thoroughly with troughing buffer 25 peg8000 in tae or tbe buffer 4 do additional electrophoresis for approximately 30 minutes until the dna band was in the middle of the well 5 collect dna in a clean tube 6 extract dna once with chloroform isoamyl alcohol 24 1 v v 7 precipitate dna with 2 volumes of ethanol at 80 c for 25 minutes 8 spin at 13 000 rpm for 30 minutes 9 discard supernatant wash dna pellet with 70 ethanol spin at 13 000 rpm for 25 minutes 10 resuspend dna in 20 μl of water or te buffer for further detail please refer to this article harnpicharnchai p etal 2007 an efficient purification and fractionation of genomic dna from soil by modified troughing method letters in applied microbiology 45 387 391 posted by arif rahman s at 19 20 no comments monday 24 november 2008 dna extraction from soil for all you guys whom dealing with environmental molecular for all you guys whom dealing with metagenomic world relax we have to be patient and keep moving forward dna extraction from soil zhou et al 1996 advantage simple high dna yield 1 weigh 5 15 g of soil into 50 ml tube 2 add 13 5 ml lysis buffer 100 mm tris hcl 100 mm sodium edta 100 mm sodium phosphate 1 5 m nacl and 1 ctab plus 100 μl of proteinase k 10 mg ml shake horizontally at 37 c for 30 minutes 3 add 1 5 ml of 20 sds incubate at 65 c for 2 hours with gentle end to end inversion every 15 20 minutes 4 spin at 7000 rpm 6000g for 10 minutes 5 transfer supernatant to a clean 50 ml tube 6 add 4 5 ml lysis buffer plus 0 5 ml of 20 sds to soil pellet vortex for 10 seconds 7 incubate at 65 c for 10 minutes 8 spin at 7000 rpm 6000g for 10 minutes 9 collect supernatant and combine with the supernatant from step5 10 repeat steps 6 9 again 11 extract the supernatant with equal volume of chloroform isoamyl alcohol 24 1 v v 12 spin at 6500 rpm 5500g for 10 minutes 13 collect aqueous top phase in a 40 ml sorvall tube 14 precipitate genomic dna with 0 6 volume of isopropanol at room temperature for 1 hour or overnight 15 spin at 11 600 rpm 16 000g for 20 minutes 16 discard supernatant wash dna pellet with approximately 3 ml of cold 70 ethanol spin at 11 600 rpm 16 000g for 10 minutes 17 air dry pellet 18 resuspend dna pellet with approximately 150 μl of dh2o ok good luck for detail please refer to this article zhou et al 1996 dna recovery from soils of diverse composition applied and environmental microbiology 62 2 316 322 posted by arif rahman s at 20 45 no comments wednesday 5 november 2008 dna extraction from kiwifruit teacher handout introduction dna is present in the cells of all living organisms this procedure is designed to extract dna from kiwi in sufficient quantity to be seen and spooled this activity is ideal for students to work in pairs but each student will have a tube of dna at the end materials knife for cutting kiwi one small ziplock bag per group of students jar or beaker that fits strainer or funnel strainer or funnel cheese cloth or a 6 coffee filter ice water bath a large mixing bowl works well water clear colored shampoo such as suave daily clarifying shampoo kiwifruit half a kiwi per group of students table salt either iodized or non iodized 1 large test tube holds 20 ml per group preferably with a cap 1 small test tube holds 10 ml for each student preferably with a cap cold 95 ethanol grain alcohol protocol 1 set up an ice water bath 2 each group will use half a kiwi and 20 ml of the following shampoo solution for one liter of the shampoo solution mix 100 ml of shampoo and 15 g of table salt add water to make a final volume of 1 liter dissolve the salt y stirring slowly to avoid foaming measure 20 ml of solution for each group of students 3 peel the kiwi cut them into about 12 pieces directions for each group 4 get 6 pieces of kiwi and put them in a ziplock bag 5 add 20 ml of shampoo solution to the ziplock bag make sure the bag is closed with out much extra air what do you think the shampoo solution does to the kiwi 6 mush the kiwi thouroughly but carefully so the bag doesn t break for about 5 minutes what does mushing the kiwi do 7 cool the kiwi mixture in the ice bath for a minute then mush the kiwi more cool then mush repeat this several times why do we cool the mixture 8 filter the mixture through cheesecloth all the groups can combine their mixtures at this point to filter together what is being filtered out what is going through the filter 9 dispense approximately 3 ml of kiwi solution into each test tube one for each student 10 being careful not to shake the tubes add approximately 2 ml of cold 95 ethanol to each tube what do you think the ethanol does why do we want it cold 11 take a look at your tube what do you see in the top portion of the liquid adapted from univ arizona posted by arif rahman s at 01 01 no comments sunday 26 october 2008 main step of metagenomic dna library posted by arif rahman s at 23 19 no comments emerging nucleic acid there are numerous microbe which have not discovered yet metagenomic is one of solution posted by arif rahman s at 22 37 no comments older posts home subscribe to posts atom may i help you custom search target kunjung tiap hari achiles apri askmsrecipe budisiswanto freedownload maninformasistrateg yudi1986 any of these interesting for you write your comment here view shoutbox shoutmix chat widget feedjit live traffic map feedjit live blog stats feedjit live traffic feed feedjit live blog stats blog archive 2016 1 february 1 samurai pena 2009 1 january 1 2008 6 december 1 november 2 october 3 about 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