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chromatography home chromatography books sitemap wikichrom mikhail tsvet about stats search solid phase extraction posted in column chromatography on dec 15 2010 sample preparation is a crucial step in any analytical method especially in chromatography where samples have to be homogenous free of interferences and safe for the column today one of the most widely used sample preparation methods is solid phase extraction spe that in the mid 1990s largely replaced the conventional but time consuming liquid liquid extraction lle the spe method resembles chromatography itself a liquid sample is passed through a packed column that selectively removes interferences or analytes the spe sample preparation technique is frequently carried out in a small disposable plastic column cartridge that looks like a 10 ml medical syringe the column is packed with a small amount 1 0 g of sorbent e g c 18 silica which is held in place by frits disposable cartridge for spe syringe style however the spe method can also be implemented by using a micro pipette tip â a 96 well plate a disk or even coated fibers the later has its own name â solid phase microextraction or spme spe is typically used to increase concentration of the analyte remove interferences for complex organic sample matrix and also to protect the column desalt in ion exchange chromatography tags adsorbent c18 solid phase extraction spe spme 908 comments chemical composition of boza posted in history on dec 09 2010 science and life magazine january 1890 1 by dr viktorina gruzdeva dec 31st 1889 during the october 20th 1889 meeting of the society for public health protection dr kazanski presented a report on the making and chemical composition of â œbozaâ russ ð ñƒð ð boza is the name of a mongolian fermented beverage made from grains a beer of sorts as you know beer is classified into 3 main categories depending on the ingredients used the first one is the oldest characterized by the absence of malt the second category is made exclusively from malt and the third kind which is something in between and requires both raw grain kernels and malted ones read more on chemical composition of boza tags beer bouza boza ð ñƒð ð malt science and life 256 comments arsenic for breakfast lunch and dinner posted in biochemistry on dec 02 2010 scanning electron micrograph of strain gfaj 1 courtesy of science aaas dr felisa wolfe simon and collegues have discovered a microorganism that can live and grow entirely off arsenic it is the first known published research that points to a bacterium that is able to use a normally highlyâ toxicâ chemical rather than the phosphate to sustain growth and life arsenic as is directly under phosphorus p in the periodic table and they both share common chemical properties however arsenic isâ normally extremely harmful to living organisms because it disrupts major metabolic pathways on the other hand arsenate aso 4 3∠the oxidized as 5 is biologically very similar to phosphate in the past scientists did find organisms thatâ canâ chemically alter arsenic and these organisms have been implicated in ground water poisoning events in bangladesh and other places in asia when people have shifted to using well water to avoid cholera read more on arsenic for breakfast lunch and dinner tags arsenic bacterium phosphate wolfe simon 350 comments rocky mountain spotted fever trends posted in biochemistry on dec 01 2010 petechial rash on my ankle caused by rmsf 2004 more than 3 years ago i shared a personal story where i â describe myâ experienceâ withâ rocky mountain spotted fever or rmsf a condition caused byâ rickettsia rickettsii a species of bacteria that is spread viaâ tick bites â that blog post has become the most commented 178 so far where people share with the worldâ theirâ horrifying accounts ofâ rmsf there is a tool by google insights for search still beta that shows web search interest for a given search term s over a period of time theâ resultsâ can be â filtered based on a geographical location and general search categories google also offers an option to forecast the search trend for yourâ queryâ about one year into the future i examined the search phrase rocky mountain spotted fever in the us from 2004 to present here is the graph click toâ enlarge rocky mountain spotted fever search trends 2004 2010 as you can see every year there is a significant spike in the number of searches around june however year after year that peak is becoming shorter and shorter forming a steadily descending trend line read more on rocky mountain spotted fever trends tags petechial rash rickettsia rickettsii rmsf rocky mountain spotted fever tick fever ticks 546 comments measuring radiation exposure from backscatter x ray posted in general science on nov 29 2010 the widespread deployment of rapiscan s body scanners in major airports around the us has raised concerns about the ionizing radiation doses to traveling public rapiscan doesn t publicly provide dose calculations but the company does claim that the x ray screening machines are in compliance with the 2002 ansi standard american national standards institute they also says that emission per scan less than 10 microrem which would translate to 0 1 âµsv but as it turns out rapiscan may haveâ significantly under calculated per scan exposure the 2002 ansi standard calls for effective radiation dose of 0 1 âµsv or less per scan per person interestingly in 2009 ansi relaxed that standard and increased the limit to 0 25 âµsv how much is 0 1 or even 0 25 âµsv well sv stands for sievert a derived si unit that measures biological absorption of ionizing radiation the national council on radiation protection and measurements recommends the following annual radiation dose limit for an individual from all radiation sources other than natural background and the individualâ s medical care is read more on measuring radiation exposure from backscatter x ray tags backscatter cesium 137 chernobyl compton iodine 131 radiation radioiodine rapiscan x ray 9 061 comments handbook of hplc 2 0 posted in hplc on nov 27 2010 amazon_link id 1574445545 target _blank amazon_link several months ago a second edition of â amazon_link id 1574445545 target _blank handbook of hplc amazon_link was released the new edition avoids well establishedâ theoreticalâ and practical aspects of hplc that were extensively covered in the first edition published in 1998 however the editors greatly update the handbook with emerging novel methods and concepts â that have contributed to the further advancement of hplc technique in the last 12 years here are someâ examples of these new aspects read more on handbook of hplc 2 0 tags book review hplc î¼hplc lcms monolithic 252 comments melamine detection in infant formula posted in hplc on nov 26 2010 in september 2008 almost 52 000 infants in china suffered urinary problems from consumption of melamine contaminated baby formulas and some other dairy products who 2009 by the end of november 2008â almost 300 000 infants were affected and six deaths have been confirmed by the chinese ministry of health a low level of melamine contamination was also found in other non dairy products made in china such as biscuits candies and even coffee drinks â reportedly more than 90 of ingested melamine is excreted with urine within 24 hours mast et al 1983 without any adverse health issues however if melamine exceeds a certain threshold it forms crystals in urine that grow into stones calculi in kidneys ureter or bladder in light of the melamine crisis the fda increased sampling and analysis of imported milk derived ingredients and products recently the government also set a threshold of 1 âµg ml for melamine in infant formula making it critical to have a low level melamine detection method there are several analytical techniques that could be used to detect and quantify melamine read more on melamine detection in infant formula tags acetonitrile c18 dad elisa gc ms hplc hplc uv infant formula melamine 620 comments pine mouth revisited posted in general science on nov 24 2010 while doing my random searches in google scholar i stumbled upon this article by marc david munk pine mouth syndrome cacogeusia following ingestion of pine nuts genus pinus an emerging problem 1 â incidentally last year i did experience pineâ mouth syndrome and wrote about it inâ cursed pine nuts or triglycerides dr munk â from department of emergency medicine read more on pine mouth revisited tags cacogeusia google trends metallogeusia pine pine nut triglycerides 971 comments backscatter x ray vs millimeter wave rf vs pat down part 1 posted in general science on nov 23 2010 yesterday i flew to san franciscoâ from new york s jfk airport â terminal 8 as i was going through the tsa security checkpoint i noticed thatâ even thoughâ osi s rapiscan secure 1000 body scanners were in place they were not used to screen the passengers relieved i happily went through a regular metalâ detector upon arrival at sfo i glanced at the security checkpoint and read more on backscatter x ray vs millimeter wave rf vs pat down part 1 tags backscatter compton explosives jfk provision rapiscan security sfo tsa x ray 260 comments ultra short gc columns posted in gas chromatography on nov 21 2010 back in 2005 two russian scientists berezkin and lapin 1 investigated the application of ultra short capillary gas chromatography gc columns ordinary the gc columns are extremely lengthy and range from 10 to 150 meters in order to achieve effective separation of mixed components berezkin sâ earlier research 2 showed that 75 of all gc columns used in scientific publications fell between 10 and 30 m length and anything shorter wasâ relativelyâ uncommon â however the authors argue for shorter column length as it may allow to lower the â column temperature during chromatographic separation which could expand the practical application of gas chromatography but on a negative side shorter columns will result in lower resolution berezkin and lapin conducted theirâ experimentsâ using hp 5890 gas chromatographâ equippedâ with an electronic pressureâ controlâ and a flameâ ionizedâ detectorâ fid the scientists used various length columns ranging from 0 1 to 5 meters to separate n c9 n c20 alkanes and showed that ultra short columns were good enough for express analysis of thermolabile and high boiling compounds the authors were optimistic of practical application of ultra short capillary columns as anâ extensionâ toâ conventionalâ gc analysis 1 v g berezkin a b lapin journal of chromatography a 1075 197 203 2005 2 v g berezkin i v malyukova zh anal khim 52 798 1997 in russian tags column gas chromatography russian 245 comments previous entries next page tag cloud adsorbent benzene china chlorophyll chromatographic chromatography column chromatography cze desi electromagnetic induction electrospray elisa eluent explosives forensic furosine gas chromatography gc ms history hplc ic isoflavones lab on a chip lcms maldi tof mass spectrometry microfluidic nano nano hplc nanoliquid nanoscale nanostream nanotechnology nanotubes news organic molecule protein rickettsia rmsf rocky mountain spotted fever science soy tcm ticks tsvet categories biochemistry column chromatography cze gas chromatography general science history hplc ic lab on a chip lcms liquid chromatography mass spectrometry nano hplc nanotech news organic chemistry reflections on science yahoo answers feed tools recent articles solid phase extraction chemical composition of boza arsenic for breakfast lunch and dinner rocky mountain spotted fever trends measuring radiation exposure from backscatter x ray handbook of hplc 2 0 melamine detection in infant formula recent entries recent comments most commented all rights reserved 2012 chromatography